Composite

Part:BBa_K3440004:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


GFP under Prhl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


Design Notes

This part was designed to test the leakiness of Prhl. A reporter GFP gene was added for that purpose. A moderate strength RBS was used (BBa_B0034)

Source

GFP from Aequorea victoria (Jellyfish)

References